Active Recombinant Human TNFSF12 Protein, Fc Tagged

• Cat.No.: TNFSF12-11241H
• Product Overview: Human TWEAK Protein, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ser 94-His 249 (Accession # O43508-1). This protein carries a human IgG1 Fc tag at the N-terminus. Predicted N-terminus: Pro

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 94-249 aa
• Description: TWEAK (tumor necrosis factor-like weak inducer of apoptosis) is a member of the TNF superfamily that controls a multitude of cellular events including proliferation, migration, differentiation, apoptosis, angiogenesis, and inflammation. TWEAK exists in both membrane-bound and secreted forms, and can induce cell apoptosis via multiple pathways of cell death in a cell type-specific manner. It has also been found to promote the proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Human TWEAK Protein, Fc Tag immobilized on CM5 Chip can bind Human TWEAK R, Fc Tag with an affinity constant of 42.8 pM as determined in a SPR assay.
• Molecular Mass: The protein has a calculated MW of 43.6 kDa. The protein migrates as 33 kDa and 48-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU/μg by the LAL method/rFC method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF12 tumor necrosis factor (ligand) superfamily, member 12 [ Homo sapiens (human) ]
• Official Symbol: TNFSF12
• Synonyms: TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; APO3L; DR3LG; TWEAK; APO3 ligand; APO3/DR3 ligand; TNF-related WEAK inducer of apoptosis; MGC20669; MGC129581
• Gene ID: 8742
• mRNA Refseq: NM_003809
• Protein Refseq: NP_003800
• MIM: 602695
• UniProt ID: O43508