Active Recombinant Human ULBP2 protein, His/Avi-tagged, Biotinylated ULBP2-051H

Active Recombinant Human ULBP2 protein, His/Avi-tagged, Biotinylated

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Active Recombinant Human ULBP2 protein, His/Avi-tagged, Biotinylated

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Cat.No. : ULBP2-051H Optional Service: Optional requirements on this protein
Product Overview : Biotinylated Recombinant Human ULBP2(Gly26-Ser217) protein, fused to his tag and Avi tag at the C-terminus, was expressed in HEK293 cells.
Description : ULBP-2 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. The name ULBP-2 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-2 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30 – 60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-2 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Our biotinylated Avi-tag ULBP-2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is minimum interference in the protein's bioactivity.
Source : HEK293 cells
Species : Human
Tag : His/Avi
Predicted N Terminal : Gly26
Form : Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Bio-activity : Measured by its binding ability in a functional ELISA. When Recombinant Human NKG2D/CD314 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human ULBP2 His-tag Avi-tag that produces 50% of the optimal binding response is approximately 175‑875 ng/mL.
Produce 50% of the optimal binding response is approximately 175‑875 ng/mL.
Molecular Mass : 30-38 kDa, under reducing conditions
Protein length : Gly26-Ser217
Endotoxin : <0.10 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Applications : Bioactivity
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 200 μg/mL in PBS.
SDS-PAGE :

2 μg/lane Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining.

Binding Activity :

Gene Name : ULBP2 UL16 binding protein 2 [ Homo sapiens ]
Official Symbol : ULBP2
Synonyms : ULBP2; UL16 binding protein 2; NKG2D ligand 2; RAET1H; N2DL-2; NKG2DL2; ALCAN-alpha; UL16-binding protein 2; retinoic acid early transcript 1H; retinoic acid early transcript 1 H; N2DL2;
Gene ID : 80328
mRNA Refseq : NM_025217
Protein Refseq : NP_079493
MIM : 605698
UniProt ID : Q9BZM5
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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