Active Recombinant Human ULBP2 protein, His/Avi-tagged, Biotinylated
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|Cat.No. :||ULBP2-051H Optional Service: Optional requirements on this protein|
|Product Overview :||Biotinylated Recombinant Human ULBP2(Gly26-Ser217) protein, fused to his tag and Avi tag at the C-terminus, was expressed in HEK293 cells.|
|Description :||ULBP-2 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. The name ULBP-2 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-2 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30 – 60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-2 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Our biotinylated Avi-tag ULBP-2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is minimum interference in the protein's bioactivity.|
|Source :||HEK293 cells|
|Predicted N Terminal :||Gly26|
|Form :||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Bio-activity :||Measured by its binding ability in a functional ELISA. When Recombinant Human NKG2D/CD314 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human ULBP2 His-tag Avi-tag that produces 50% of the optimal binding response is approximately 175‑875 ng/mL.
Produce 50% of the optimal binding response is approximately 175‑875 ng/mL.
|Molecular Mass :||30-38 kDa, under reducing conditions|
|Protein length :||Gly26-Ser217|
|Endotoxin :||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity :||>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage :||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.|
|Reconstitution :||Reconstitute at 200 μg/mL in PBS.|
2 μg/lane Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining.
|Binding Activity :||
|Gene Name :||ULBP2 UL16 binding protein 2 [ Homo sapiens ]|
|Official Symbol :||ULBP2|
|Synonyms :||ULBP2; UL16 binding protein 2; NKG2D ligand 2; RAET1H; N2DL-2; NKG2DL2; ALCAN-alpha; UL16-binding protein 2; retinoic acid early transcript 1H; retinoic acid early transcript 1 H; N2DL2;|
|Gene ID :||80328|
|mRNA Refseq :||NM_025217|
|Protein Refseq :||NP_079493|
|UniProt ID :||Q9BZM5|
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