- Human(18)
Active Recombinant Human ULBP2 protein, His/Avi-tagged, Biotinylated
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Cat.No. : | ULBP2-051H Optional Service: Optional requirements on this protein |
Product Overview : | Biotinylated Recombinant Human ULBP2(Gly26-Ser217) protein, fused to his tag and Avi tag at the C-terminus, was expressed in HEK293 cells. |
Description : | ULBP-2 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. The name ULBP-2 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-2 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30 – 60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-2 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Our biotinylated Avi-tag ULBP-2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is minimum interference in the protein's bioactivity. |
Source : | HEK293 cells |
Species : | Human |
Tag : | His/Avi |
Predicted N Terminal : | Gly26 |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Bio-activity : | Measured by its binding ability in a functional ELISA. When Recombinant Human NKG2D/CD314 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human ULBP2 His-tag Avi-tag that produces 50% of the optimal binding response is approximately 175‑875 ng/mL. Produce 50% of the optimal binding response is approximately 175‑875 ng/mL. |
Molecular Mass : | 30-38 kDa, under reducing conditions |
Protein length : | Gly26-Ser217 |
Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Applications : | Bioactivity |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 200 μg/mL in PBS. |
SDS-PAGE : |
![]() 2 μg/lane Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining. |
Binding Activity : |
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Gene Name : | ULBP2 UL16 binding protein 2 [ Homo sapiens ] |
Official Symbol : | ULBP2 |
Synonyms : | ULBP2; UL16 binding protein 2; NKG2D ligand 2; RAET1H; N2DL-2; NKG2DL2; ALCAN-alpha; UL16-binding protein 2; retinoic acid early transcript 1H; retinoic acid early transcript 1 H; N2DL2; |
Gene ID : | 80328 |
mRNA Refseq : | NM_025217 |
Protein Refseq : | NP_079493 |
MIM : | 605698 |
UniProt ID : | Q9BZM5 |
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◆ Lysates
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