| Cat.No. : |
ULBP2-051H
Optional Service: Optional requirements on this protein
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| Product Overview : |
Biotinylated Recombinant Human ULBP2(Gly26-Ser217) protein, fused to his tag and Avi tag at the C-terminus, was expressed in HEK293 cells. |
| Description : |
ULBP-2 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. The name ULBP-2 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-2 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30 – 60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-2 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Our biotinylated Avi-tag ULBP-2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is minimum interference in the protein's bioactivity. |
| Source : |
HEK293 cells |
| Species : |
Human |
| Tag : |
His/Avi |
| Predicted N Terminal : |
Gly26 |
| Form : |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Bio-activity : |
Measured by its binding ability in a functional ELISA. When Recombinant Human NKG2D/CD314 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human ULBP2 His-tag Avi-tag that produces 50% of the optimal binding response is approximately 175‑875 ng/mL.
Produce 50% of the optimal binding response is approximately 175‑875 ng/mL. |
| Molecular Mass : |
30-38 kDa, under reducing conditions |
| Protein length : |
Gly26-Ser217 |
| Endotoxin : |
<0.10 EU per 1 μg of the protein by the LAL method. |
| Purity : |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Applications : |
Bioactivity |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution : |
Reconstitute at 200 μg/mL in PBS. |
| SDS-PAGE : |
2 μg/lane Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining.
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| Binding Activity : |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.