Active Recombinant Human ULK1 Protein, 1-314, N-6×His tagged

• Cat.No.: ULK1-13H
• Product Overview: Recombinant human ULK1 (amino acids 1-314) with N-terminal 6×His-tag was expressed by baculovirus in Sf21 insect cells, purified using immobilized metal affinity chromatography followed by gel filtration.

Specification

• Species: Human
• Source: Insect cells
• Tag: His
• Protein Length: 1-314
• Description: Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; positive regulation by symbiont of host autophagy; and protein phosphorylation. Located in autophagosome; cytosol; and phagophore assembly site membrane. Is extrinsic component of autophagosome membrane; extrinsic component of omegasome membrane; and extrinsic component of phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex.
• Form: Frozen solution
• Bio-activity: 2858 U/mg, where one unit of ULK1 activity is defined as 1 nmol phosphate incorporated into 2 mg/mL casein per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 39 kDa
• AA Sequence: MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLAS
• Purity: 83% by SDS-PAGE and Coomassie blue staining
• Applications: Drug Discovery and Development
• Storage: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.
• Concentration: 0.45 mg/mL
• Storage Buffer: 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol.

Gene Information

• Gene Name: ULK1 unc-51 like autophagy activating kinase 1 [ Homo sapiens (human) ]
• Official Symbol: ULK1
• Synonyms: ULK1; unc-51 like autophagy activating kinase 1; ATG1; ATG1A; UNC51; hATG1; Unc51.1; serine/threonine-protein kinase ULK1; ATG1 autophagy related 1 homolog; autophagy-related protein 1 homolog; EC 2.7.11.1
• Gene ID: 8408
• mRNA Refseq: NM_003565
• Protein Refseq: NP_003556
• MIM: 603168
• UniProt ID: O75385