| Species : |
Human |
| Source : |
Insect cells |
| Tag : |
His |
| Protein Length : |
1-314 |
| Description : |
Enables identical protein binding activity; protein serine/threonine kinase activity; and small GTPase binding activity. Involved in several processes, including autophagosome assembly; positive regulation by symbiont of host autophagy; and protein phosphorylation. Located in autophagosome; cytosol; and phagophore assembly site membrane. Is extrinsic component of autophagosome membrane; extrinsic component of omegasome membrane; and extrinsic component of phagophore assembly site membrane. Part of Atg1/ULK1 kinase complex. |
| Form : |
Frozen solution |
| Bio-activity : |
2858 U/mg, where one unit of ULK1 activity is defined as 1 nmol phosphate incorporated into 2 mg/mL casein per minute at 30 centigrade with a final ATP concentration of 100 μM. |
| Molecular Mass : |
39 kDa |
| AA Sequence : |
MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLAS |
| Purity : |
83% by SDS-PAGE and Coomassie blue staining |
| Applications : |
Drug Discovery and Development |
| Storage : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
| Concentration : |
0.45 mg/mL |
| Storage Buffer : |
50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. |
