Active Recombinant Human USP22 protein, His-tagged

• Cat.No.: USP22-12H
• Product Overview: Recombinant Human USP22 protein with his tag at N-terminal was expressed in Sf 21 (baculovirus).

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-525 aa
• Description: Ubiquitin specific peptidase 22 is a protein that in humans is encoded by the USP22 gene on chromosome 17. USP22 is known to function as a histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA.
• Bio-activity: Recombinant human USP22 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP22 concentration of 20-100 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550, U-555) substrates.
• Molecular Mass: 59 kDa
• Purity: >95%, by SDS-PAGE under reducing conditions
• Notes: Recombinant USP22 is not active toward poly-Ubiquitin chains, Ubiquitinated histones and other Ubiquitinated protein substrates in the absence of adapter proteins such as ATXN7L or ENY2.
• Storage: 12 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: USP22 ubiquitin specific peptidase 22 [ Homo sapiens (human) ]
• Official Symbol: USP22
• Synonyms: Deubiquitinating enzyme 22; EC 3.1.2.15; EC 3.4.19.12; KIAA1063ubiquitin carboxyl-terminal hydrolase 22; KIAA1064; ubiquitin specific peptidase 22; ubiquitin specific peptidase 3-like; ubiquitin specific protease 22; Ubiquitin Thioesterase 22; Ubiquitin thiolesterase 22; ubiquitin-specific processing protease 22; Ubiquitin-specific-processing protease 22; USP22; USP3L
• Gene ID: 23326
• mRNA Refseq: NM_015276
• Protein Refseq: NP_056091
• MIM: 612116
• UniProt ID: Q9UPT9
• Chromosome Location: 17p11.2
• Pathway: Chromatin modifying enzymes, organism-specific biosystem (from REACTOME); Chromatin organization, organism-specific biosystem (from REACTOME); Deubiquitination, organism-specific biosystem (from REACTOME)
• Function: contributes_to H4 histone acetyltransferase activity; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding