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Recombinant Human USP22 293 Cell Lysate

Cat.No. : USP22-464HCL
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Description : Antigen standard for ubiquitin specific peptidase 22 (USP22) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : USP22 ubiquitin specific peptidase 22 [ Homo sapiens ]
Official Symbol : USP22
Synonyms : USP22; ubiquitin specific peptidase 22; ubiquitin specific peptidase 3 like , ubiquitin specific protease 22 , USP3L; ubiquitin carboxyl-terminal hydrolase 22; KIAA1063; ubiquitin thioesterase 22; deubiquitinating enzyme 22; ubiquitin thiolesterase 22; ubiquitin specific protease 22; ubiquitin-specific processing protease 22; ubiquitin-specific-processing protease 22; USP3L;
Gene ID : 23326
mRNA Refseq : NM_015276
Protein Refseq : NP_056091
MIM : 612116
UniProt ID : Q9UPT9
Chromosome Location : 17p11.2

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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