Active Recombinant Human VEGFB protein, hFc-tagged

• Cat.No.: VEGFB-275H
• Product Overview: Active Recombinant Human VEGFB protein(NP_003368.1) (Pro22-Ala207) was expressed in HEK293 with the Fc region of human IgG1 at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 22-207 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human S4-Fc3L3-VEGFB at 10 μg/ml (100 μL/well) can bind biotinylated human NRP1-Fc, The EC50 of biotinylated human NRP1-Fc is 0.03-0.07μg/mL.
• Molecular Mass: The recombinant human VEGFB consists 446 amino acids and predicts a molecular mass of 47.8 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > (75.2+22) % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VEGFB vascular endothelial growth factor B [ Homo sapiens ]
• Official Symbol: VEGFB
• Synonyms: VEGFB; vascular endothelial growth factor B; VRF; VEGFL; VEGF-related factor
• Gene ID: 7423
• mRNA Refseq: NM_001243733
• Protein Refseq: NP_001230662
• MIM: 601398
• UniProt ID: P49765