||Vitronectin is a secreted glycoprotein detected as a mixture of 75 kilodalton ( kDa) and 65 kDa polypeptides in serum and the extracellular matrix. It primarily in monomeric form, but it can also be found as a dimer after endogenous cleavage. Vitronectin can support cell adhesion and spreading through binding to various integrins and other proteoglycans. The cell-binding site of vitronectin involves a common cell-attachment tripeptide Arg-Gly-Asp Additionally, recombinant vitronectin can function as a chemically-defined matrix component in human embryonic stem cell and induced pluripotent stem cell.
||Recombinant Human Vitronectin promotes attachment of adherent cell in serum free, such as hESC and iPSC. Measured by the ability of the immobilized protein to support the adhesion of HUVEC cells, when 5 x 104 cells/well are added to Vitronectin coated plates (5 μg/mL with 100 μL/well), approximately >60% will adhere after 30 minutes at 37 centigrade.
|Molecular Mass :
||~75 kDa homotrimer
||<0.5 EU/ μg
||High Purity, >95% by SDS-PAGE
||Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -80 centigrade under sterile conditions after reconstitution.
|Storage Buffer :
||Lyophilized from a 0.2 μm filtered solution in PBS without carrier protein
||Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.