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Active Recombinant MERS Spike RBD protein, His-tagged

Cat.No. : Spike-4733M
Product Overview : Recombinant MERS protein(Glu 367 - Tyr 606), fused with His tag, was expressed in HEK293.
  • Specification
  • Gene Information
  • Related Products
Source : HEK293
Species : MERS
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human DPPIV, Fc Tag at 5 μg/mL (100 μL/well) can bind MERS S protein RBD, His Tag with a linear range of 0.6-5 ng/mL.Immobilized MERS S protein RBD, His Tag at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.313-2.5 μg/mL.
Molecular Mass : This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 28.2 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein Length : Glu 367 - Tyr 606
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
Official Symbol : Spike
Synonyms : Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron
Gene ID : 43740568
Protein Refseq : YP_009724390.1
UniProt ID : P0DTC2

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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