Active Recombinant Mouse Angpt2 Protein, His-tagged

• Cat.No.: Angpt2-587M
• Product Overview: Recombinant Mouse Angpt2 (Accession # NP_031452) Met1-Phe496, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Species: Mouse
• Source: CHO
• Tag: His
• Protein Length: Met1-Phe496
• Predicted N Terminal: Asp68
• Form: Lyophilized from a 0.2 µm filtered solution in HEPES, NaCl and CHAPS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to activate Tie-2 in C6 rat glial cells transfected with human Tie-2.1 µg/mL of Recombinant Mouse Angiopoietin-2 significantly induces phosphorylation of human Tie-2 in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.
• Molecular Mass: Recombinant Mouse Angpt2, His-tagged has a calculated MW of 49.7 kDa. In SDS-PAGE migrates as 60-66 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 200 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Angpt2 angiopoietin 2 [ Mus musculus ]
• Official Symbol: Angpt2
• Synonyms: ANGPT2; angiopoietin 2; angiopoietin-2; Ang2; Agpt2; Ang-2
• Gene ID: 11601
• mRNA Refseq: NM_007426
• Protein Refseq: NP_031452