Active Recombinant Mouse Bid protein(Met1-Asp195), His&GST-tagged

Cat.No. : Bid-4029M
Product Overview : Recombinant Mouse BID (EDK99650.1) (Met 1-Asp 195) was expressed in E. coli, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Source : E. coli
Species : Mouse
Tag : N-His&GST
Protein length : Met1-Asp195
Form : Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.8. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse BID at 10 μg/mL (100 μl/well) can bind biotinylated human BCL2L1, The EC50 of biotinylated human BCL2L1 is 7.01 ng/mL. 3. Immobilized mouse BID at 10 μg/mL (100 μl/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 7.1 ng/mL.
Molecular Mass : The recombinant mouse BID/GST chimera consisting of 435 amino acids and has a calculated molecular mass of 50 kDa. It migrates as a 47 kDa band in SDS-PAGE under reducing conditions.
Purity : > 95 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Bid BH3 interacting domain death agonist [ Mus musculus ]
Official Symbol : Bid
Synonyms : BID; BH3 interacting domain death agonist; BH3-interacting domain death agonist; p22 BID; AI875481; AU022477; 2700049M22Rik;
Gene ID : 12122
mRNA Refseq : NM_007544
Protein Refseq : NP_031570

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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In which cellular processes does the BID protein play? 06/24/2022

BID protein mainly plays a role in the process of apoptosis. It can regulate cell survival and death signaling by interacting with other members of the Bcl-2 family.

Can the expression level and activity of BID protein be determined? 11/20/2020

The expression level of BID protein can be detected by immunohistochemistry, western blotting, real-time quantitative PCR and other techniques. At the same time, the activity of BID protein can be assessed by detecting the level of phosphorylation of BID protein and the number of BID protein within cells.

How are BID proteins involved in the process of apoptosis? 11/05/2020

During apoptosis, the BID protein is activated, forming complexes with other Bcl-2 family members, releasing cytochrome c, initiating a cascade of caspase enzymes, and ultimately leading to apoptosis.

How are the interactions between BID proteins and other proteins regulated? 09/21/2020

The interaction between BID protein and other proteins is regulated by a variety of factors, including phosphorylation, methylation, acetylation and other modifications, as well as interactions with other proteins. These factors can affect the interaction between BID protein and other proteins, which in turn affects the regulatory process of apoptosis.

How can BID proteins be used as biomarkers in clinical practice? 08/29/2020

In clinical practice, the expression level of BID protein can be measured to predict disease progression and prognosis, and to guide the choice of treatment regimen. For example, in some tumor diseases, measuring the expression level of BID protein can help doctors determine the type, stage, and prognosis of the disease, and guide the choice of treatment regimen.

Can the expression level of BID protein be regulated? 06/12/2020

Expression level of BID protein is regulated by a variety of factors, including gene transcription, translation, modification, and degradation. These factors can affect the expression level of BID protein, which in turn affects the survival and death signals of cells.

Customer Reviews (3)

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Reviews
12/19/2021

    Activity retention time is very long and can be maintained for a long time.

    09/13/2021

      The detection method is very sensitive and can easily detect small amounts of protein.

      04/10/2021

        Expression level and stability are good and will be repurchased.

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