Active Recombinant Mouse Car4 protein(Met1-Ser277), His-tagged

• Cat.No.: Car4-662M
• Product Overview: Recombinant Mouse Car4 (NP_031633.1) without the C-terminal propeptide (Met 1-Ser 277) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Protein length: Met1-Ser277
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its esterase activity. The specific activity is >10 pmoles/min/μg.
• Molecular Mass: The secreted recombinant mouse Car4 consists of 271 amino acids and has a calculated molecular mass of 31 kDa as estimated in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Car4 carbonic anhydrase 4 [ Mus musculus ]
• Official Symbol: Car4
• Synonyms: CAR4; carbonic anhydrase 4; CA IV; CA-IV; carbonic anhydrase IV; carbonate dehydratase IV; Ca4; AW456718
• Gene ID: 12351
• mRNA Refseq: NM_007607
• Protein Refseq: NP_031633

Reviews

04/14/2022

Reliable partner for protein expression and analysis.

04/12/2022

Cost-effective quantitation services, a great research investment.

12/09/2020

Invaluable for our protein analysis and quantification endeavors.

Q&As

What role does CA4 play in the respiratory system?

CA4 assists in CO2 and bicarbonate regulation, pivotal for efficient respiratory gas exchange.

Which tissues or cell types predominantly express CA4?

CA4 is highly expressed in the kidney and red blood cells.

What is the primary physiological function of CA4?

CA4 catalyzes the reversible hydration of carbon dioxide, helping regulate pH and CO2 transport.

What are the known post-translational modifications on CA4?

Typical modifications like phosphorylation can be identified using techniques such as mass spectrometry.

Is CA4 implicated in any specific cellular signaling pathways?

While primarily enzymatic, any signaling roles for CA4 would require deeper investigation.

Are there known diseases or conditions associated with CA4 mutations or dysregulation?

CA4 dysregulation could relate to renal disorders or hematological abnormalities, though research is ongoing.

How does the enzymatic activity of CA4 compare to other carbonic anhydrase family members?

CA4, like other isoforms, is involved in CO2 hydration, but its specific efficiency and affinity might vary.