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Active Recombinant Mouse Casp3 protein

Cat.No. : Casp3-1643M
Product Overview : Recombinant Mouse Casp3 protein was expressed in E. coli.
  • Specification
  • Gene Information
  • Related Products
Source : E. coli
Species : Mouse
Bio-activity : 300000 units/mg
Molecular Mass : Of large (17 kD) and small (11 kD) subunits
Unit Definition : One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Applications : Active caspase-3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
Notes : Centrifuge the vial prior to opening.
Storage : The lyophilized caspase-3 is stable for 1 year at –70 centigrade. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70 centigrade. Avoid multiple freeze/thaw cycles as activity might decrease.
Reconstitution : Reconstitute to 1 unit per µl in PBS containing 15% glycerol
Gene Name : Casp3 caspase 3 [ Mus musculus ]
Official Symbol : Casp3
Synonyms : CASP3; caspase 3; caspase-3; SCA-1; CASP-3; CPP-32; protein Yama; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis related cysteine protease; CC3; AC-3; Lice; Yama; mldy; CPP32; Apopain; Caspase-3; A830040C14Rik;
Gene ID : 12367
mRNA Refseq : NM_009810
Protein Refseq : NP_033940
Pathway : Activation of DNA fragmentation factor, organism-specific biosystem; Activation of caspases through apoptosome-mediated cleavage, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem
Function : aspartic-type endopeptidase activity; cyclin-dependent protein kinase inhibitor activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; cysteine-type peptidase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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