Recombinant Mouse CCL22 Protein

• Cat.No.: CCL22-2887M
• Product Overview: Fractalkine Mouse Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 76 amino acids and having a molecular mass of 8.7 kDa. The CX3CL1 is purified by proprietary chromatographic techniques.

Specification

• Description: Fractalkine soluble form is chemotactic for t-cells and monocytes, but not for neutrophils. Fractalkine membrane-bound form promotes adhesion of those leukocytes to endothelial cells. Fractalkine regulates leukocyte adhesion and migration processes at the endothelium and binds to CX3CR1. Natural Human Fractalkine is produced as a long protein (373-amino acid) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the cell surface. Fractalkine gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22.
• Source: E. coli
• Species: Mouse
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: The ED50 as determined by a cell proliferation assay using human peripheral blood lymphocytes (PBL) is less than 0.5 μg/mL, corresponding to a specific activity of > 2000 IU/mg.
• Molecular Mass: 8.7 kDa
• AA Sequence: QHLGMTKCEIMCGKMTSRIPVALLI RYQLNQESCGKRAIVLETTQHRRFC ADPKEKWVQDAMKHLDHQAAALTKN G.
• Purity: Greater than 97.0% as determined by:
  (a) Analysis by RP-HPLC.
  (b) Analysis by SDS-PAGE.
• Stability: Lyophilized CX3CL1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution CX3CL1 should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade.
  For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  Please prevent freeze-thaw cycles.
• Usage: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
• Storage Buffer: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Shipping: Shipped at Room temp.
• Reconstitution: It is recommended to reconstitute the lyophilized CX3CL1 in sterile 18MΩ-cm H2O not less than 100 μg/mL, which can then be further diluted to other aqueous solutions.

Gene Information

• Gene Name: Ccl22 chemokine (C-C motif) ligand 22 [ Mus musculus ]
• Official Symbol: CCL22
• Synonyms: CCL22; chemokine (C-C motif) ligand 22; C-C motif chemokine 22; CC chemokine ABCD-1; small-inducible cytokine A22; activated B and dendritic cell-derived; small inducible cytokine subfamily A22; dendritic cell and B cell derived chemokine; small inducible cytokine subfamily A, member 22; SMALL INDUCIBLE CYTOKINE A22 PRECURSOR (CC CHEMOKINE ABCD-1) (ACTIVATED B AND DENDRITIC CELL-DERIVED); MDC; DCBCK; ABCD-1; Scya22
• Gene ID: 20299
• mRNA Refseq: NM_009137
• Protein Refseq: NP_033163
• UniProt ID: O88430

Reviews

05/01/2022

The catalytic activity of CCL22 is very strong, which can significantly improve the selectivity and yield of chemical reactions, and has high application value.

03/13/2022

This repeatability of CCL22 is very good, which can ensure the safety of the experiment and avoid unnecessary risks.

08/25/2019

CCL22 were well suited for our research needs due to their simple storage conditions and long-term preservation.

Q&As

What are the gene expression regulatory mechanisms of CCL22 protein?

The expression of CCL22 gene is regulated by a variety of signaling pathways, including inflammatory factor-mediated signaling pathways, cytokine-mediated signaling pathways, and transcription factors.

What is the potential application value of CCL22 protein in treatment?

Studies have shown that by interfering with the interaction of the CCL22 protein and its receptor CCR4, tumor growth, improve inflammatory response, and regulate autoimmune diseases can be inhibited. These findings provide potential application value for the development of corresponding therapeutic strategies.

Are there inhibitors or antagonists that interact with the CCL22 protein?

A number of inhibitors or antagonists that interact with CCL22 proteins have been developed that block the binding of CCL22 to its receptor CCR4, thereby interfering with its biological function.

How to study the mechanism of action of CCL22 protein in specific diseases?

The mechanism of action of CCL22 protein in specific diseases can be studied through cell culture experiments, animal models, clinical samples, and related biochemical techniques.

How does the CCL22 protein relate to other chemokines?

There are complex interactions between CCL22 proteins and other chemokines. They may influence the migration and localization of immune cells by competing for receptors, mutual regulation of expression, etc.

What is the role of CCL22 protein in tumor immunotherapy?

Some studies have shown that in tumor immunotherapy, inhibiting the expression of CCL22 or interfering with its interaction with CCR4 can enhance the effect of immunotherapy and improve the survival rate of patients.