Active Recombinant Mouse Ccl4 Protein (Ala24-Asn92), N-His tagged, Animal-free, Carrier-free

• Cat.No.: Ccl4-77M
• Product Overview: Recombinant Mouse Ccl4 Protein (Ala24-Asn92) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: C-C Motif Chemokine Ligand 4 (CCL4) is a 7.66 kDa cytokine with 69 amino acid residues. CCL4, also named macrophage inflammatory protein-1β (MIP-1β), is mainly secreted from neutrophils, monocytes, B cells, T cells, fibroblasts, endothelial cells, and epithelial cells. In addition, CCL4 participates in immune responses, including recruitment of immune cells like lymphocytes, monocytes, and leukocytes, response to IL-1 and IFNγ, and production of TNF when CCL4 binds to CCR5.
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to chemoattract human PBMCs using a concentration range of 20.0 - 200.0 ng/mL. Note: Results may vary from different PBMC donors.
• Molecular Mass: The protein has a calculated MW of 8.64 kDa.
  The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Ala24-Asn92
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Ccl4 chemokine (C-C motif) ligand 4 [ Mus musculus (house mouse) ]
• Official Symbol: Ccl4
• Synonyms: CCL4; chemokine (C-C motif) ligand 4; C-C motif chemokine 4; ACT2; SIS-gamma; MIP-1 beta; MIP-1-beta; immune activation protein 2; small inducible cytokine A4; small-inducible cytokine A4; macrophage inflammatory protein 1-beta; Act-2; Mip1b; Scya4; MIP-1B; AT744.1
• Gene ID: 20303
• mRNA Refseq: NM_013652
• Protein Refseq: NP_038680
• UniProt ID: P14097