Species : |
Mouse |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
Met1-Glu245 |
Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : |
Measured by its binding ability in a functional ELISA. Immobilized mouse CD86 at 20 μg/ml (100 μl/well) can bind mouse CD28 with a linear range of 6.4-800 ng/ml. |
Molecular Mass : |
The recombinant mouse CD86 consists of 233 amino acids after removal of the signal peptide and has a predicted molecular mass of 26.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD86 is approximately 40-55 kDa due to glycosylation. |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : |
> 92 % as determined by SDS-PAGE |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |