| Species : |
Mouse |
| Source : |
Mammalian Cells |
| Tag : |
Non |
| Description : |
Cadherin 1, type 1, E-cadherin (epithelial), also known as CDH1 and CD32, is a tumor suppressor gene. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. E-cadherin-mediated cell-cell adhesion pathway may represent a novel chemotherapeutic target for bladder cancer, prostate cancer, and renal-cell carcinoma. |
| Form : |
Lyophilized |
| Bio-activity : |
The biological activity of Mouse E-CAD was determined by the ability of the immobilized protein to support the adhesion of the MCF7 human breast adenocarcinoma cells. When 5 x 10^4 cells/well are added to rmECAD/Fc Chimera coated plates (1.5 μg/ml with 100 μl/well), approximately 50-70% will adhere after 90 minutes at room temperature.
Each investigator should determine their own optimal working dilution for specific applications. |
| Molecular Mass : |
The predicted molecular weight of Recombinant Mouse ECAD is Mr 88.2 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 120 kDa. |
| AA Sequence : |
dwvippiscpenekgefpknlvqiksnrdketkvfysitgqgadkppvgvfiieretgwlkvtqpldreaiakyilyshavssngeavedpmeivitvtdqndnrpeftqevfegsvaegavpgtsvmkvsatdadddvntynaaiaytivsqdpelphknmftvnrdtgvisvltsgldresyptytlvvqaadlqgeglsttakavitvkdindnapvfnpstyqgqvpenevnariatlkvtdddapntpawkavytvvndpdqqfvvvtdpttndgilktakgldfeakqqyilhvrveneepfegslvpstatvtvdvvdvneapifmpaerrvevpedfgvgqeitsytarepdtfmdqkityriwrdtanwleinpetgaiftraemdredaehvknstyvaliiatddgspiatgtgtlllvlldvndnapipeprnmqfcqrnpqphiitildpdlppntspftaelthgasvnwtieyndaaqeslilqprkdleigeykihlkladnqnkdqvttldvhvcdcegtvnncmkagivaaglqviegrmdpkscdkthtcppcpapellggpsvflfppkpkdtlmisrtpevtcvvvdvshedpevkfnwyvdgvevhnaktkpreeqynstyrvvsvltvlhqdwlngkeykckvsnkalpapiektiskakgqprepqvytlppsrdeltknqvsltclvkgfypsdiavewesngqpennykttppvldsdgsfflyskltvdksrwqqgnvfscsvmhealhnhytqkslslspgkhhhhhh |
| Endotoxin : |
<1.0 EU/μg as determined by the LAL method |
| Purity : |
>90% by SDS-PAGE and analyzed by silver stain. |
| Notes : |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Storage : |
This lyophilized protein is stable for six to twelve months when stored desiccated at -20 centigrade to -70 centigrade. After aseptic reconstitution, this protein may be stored at 2 centigrade to 8 centigrade for one month or at -20 centigrade to -70 centigrade in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
| Storage Buffer : |
This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution containing 50mM Tris-Citrate, 50 mM NaCl and 2 mM CaCl2, pH 6.5. |
