Active Recombinant Mouse Chemokine (C-C Motif) Ligand 8

• Cat.No.: Ccl8-285M
• Product Overview: Recombinant Mouse MCP-2, also known as CCL8, produced in E.coli is a non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8,504 Daltons.

Specification

• Cat. No.: Ccl8-285M
• Description: Chemokine (C-C motif) ligand 8, also known as monocyte chemoattractant protein 2 (MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids.
• Species: Mouse
• Source: E.coli
• Form: Lyophilized with no additives.
• Purity: Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Bio-activity: MCP-2 is fully biologically active when compared to standard. The specific activity as determined by the ability of MCP-2to chemoattract human peripheral blood at 10-100ng/ml.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
• Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
• Amino acid sequence: GPDKAPVTCC FHVLKLKIPL RVLKSYERIN NIQCPMEAVVQTKQGMSLC VDPTQKWVSE YMEILDQKSQ ILQP
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Gene Information

• Gene Name: Ccl8 chemokine (C-C motif) ligand 8 [ Mus musculus ]
• Official Symbol: Ccl8
• Synonyms: Ccl8; chemokine (C-C motif) ligand 8; HC14; Mcp2; MCP-2; Scya8; AB023418; OTTMUSP00000000873; small inducible cytokine A8; monocyte chemoattractant protein-2; 1810063B20Rik
• Gene ID: 20307
• mRNA Refseq: NM_021443
• Protein Refseq: NP_067418
• UniProt ID: Q9Z121
• Chromosome Location: 47.0 cM
• Pathway: 1810063B20Rik; Chemokine signaling pathway; Cytokine-cytokine receptor interaction
• Function: chemokine activity; cytokine activity; heparin binding