Active Recombinant Mouse Csf1 Protein (Lys33-Pro187), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Csf1-18M
• Product Overview: Recombinant Mouse Csf1 Protein (Lys33-Pro187) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Macrophage Colony-Stimulating Factor (M-CSF) is a 19.02 kDa hematopoietic Growth Factors with 162 amino acid residues. The active form of the protein is homodimer, and secreted by osteoblasts. M-CSF controls the production, differentiation, and function of monocytes, macrophages, and bone marrow progenitor cells. M-CSF is able to activate CSF-1 signaling pathway via binding its receptor CSF-1R.
• Source: E. coli
• Species: Mouse
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in NFS-60 cells. The ED50 for this effect is < 2 ng/mL. The specific activity of recombinant mouse M-CSF is approximately >5 × 10⁵ IU/mg.
• Molecular Mass: The protein has a calculated MW of 19.02 kDa.
  The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Lys33-Pro187
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 2 weeks under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Csf1 colony stimulating factor 1 (macrophage) [ Mus musculus (house mouse) ]
• Official Symbol: Csf1
• Synonyms: CSF1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; osteopetrosis; op; Csfm; MCSF; C87615
• Gene ID: 12977
• mRNA Refseq: NM_001113529
• Protein Refseq: NP_001107001
• UniProt ID: P07141