Active Recombinant Mouse Csf1 Protein (Lys33-Pro187), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Csf1-18M
• Product Overview: Recombinant Mouse Csf1 Protein (Lys33-Pro187) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Macrophage Colony-Stimulating Factor (M-CSF) is a 19.02 kDa hematopoietic Growth Factors with 162 amino acid residues. The active form of the protein is homodimer, and secreted by osteoblasts. M-CSF controls the production, differentiation, and function of monocytes, macrophages, and bone marrow progenitor cells. M-CSF is able to activate CSF-1 signaling pathway via binding its receptor CSF-1R.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in NFS-60 cells. The ED50 for this effect is < 2 ng/mL. The specific activity of recombinant mouse M-CSF is approximately >5 × 10⁵ IU/mg.
• Molecular Mass: The protein has a calculated MW of 19.02 kDa.
  The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Lys33-Pro187
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 2 weeks under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Csf1 colony stimulating factor 1 (macrophage) [ Mus musculus (house mouse) ]
• Official Symbol: Csf1
• Synonyms: CSF1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; osteopetrosis; op; Csfm; MCSF; C87615
• Gene ID: 12977
• mRNA Refseq: NM_001113529
• Protein Refseq: NP_001107001
• UniProt ID: P07141

Reviews

10/30/2017

The CSF1 protein is renowned for its exceptional quality and reliability, making it an ideal choice to fulfill my experimental requirements.

03/10/2017

With its high purity and stability, this protein ensures dependable and accurate results, which are crucial for the success of my research endeavors.

06/09/2016

Whether it is for investigating cellular pathways, studying protein functions, or exploring signaling cascades, the CSF1 protein offers unparalleled versatility and performance.

Q&As

How does CSF1 affect cancer progression?

CSF1 is implicated in tumor-associated macrophages, influencing tumor growth, invasion, and the tumor microenvironment.

What are the potential side effects of CSF1 therapy?

Side effects may include immune-related adverse events, inflammation, and alterations in white blood cell counts.

Can CSF1 therapy be combined with other treatments?

Yes, there is ongoing research on combining CSF1-targeted therapies with other immunotherapies or conventional treatments to enhance efficacy.

What are the challenges in developing CSF1-based therapies?

Challenges include maintaining the balance of immune responses, potential off-target effects, and variability in patient responses.

In what ways is CSF1 being researched for neurological disorders?

Studies are exploring its role in neuroinflammation, neuroprotection, and potential therapeutic interventions for conditions like Alzheimer's disease.