Species : |
Mouse |
Source : |
E.coli |
Protein Length : |
125 |
Description : |
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine and other immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor that activates effector functions of granulocytes, monocytes/macrophages and eosinophils. |
Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Bio-activity : |
ED50 < 5 pg/mL, measured in a cell proliferation assay using mouse FDC-P1 cells, corresponding to a specific activity of >2 8 units/mg. |
Molecular Mass : |
14.3 kDa, observed by reducing SDS-PAGE. |
AA Sequence : |
MAPTRSPITVTRPWKHVEAIKEALNLLDDMPVTLNEEVEVVSNEFSFKKLTCVQTRLKIFEQGLRGNFTKLKGALNMTASYYQTYCPPTPETDCETQVTTYADFIDSLKTFLTDIPFECKKPVQK |
Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
Purity : |
> 98% as analyzed by SDS-PAGE&HPLC. |
Storage : |
Lyophilized recombinant Mouse GM-CSF remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, Mouse GM-CSF should be stable up to 1 week at 4 centigrade or up to 3 months at -20 centigrade. |
Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |