Active Recombinant Mouse Fgf1

• Cat.No.: Fgf1-634M
• Product Overview: Recombinant Mouse Fgf1 (Accession # NP_034327) Phe16-Asp155, fused with an N-terminal Met, was produced in E. coli.

Specification

• Source: E. coli
• Species: Mouse
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120.The ED50 for this effect is typically 0.05 - 0.25 ng/mL in the presence of 10 µg/mL heparin.
• Molecular Mass: Recombinant Mouse Fgf1, Met-tagged has a calculated MW of 16 kDa.
• Protein length: Phe16-Asp155
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 200 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Fgf1 fibroblast growth factor 1 [ Mus musculus ]
• Official Symbol: Fgf1
• Synonyms: FGF1; fibroblast growth factor 1; aFGF; HBGF-1; acidic fibroblast growth factor; heparin-binding growth factor 1; fibroblast growth factor 1 (acidic); Fam; Fgfa; Dffrx; Fgf-1
• Gene ID: 14164
• mRNA Refseq: NM_010197
• Protein Refseq: NP_034327