| Species : |
Mouse |
| Source : |
E. coli |
| Tag : |
His |
| Protein Length : |
189-303 aa |
| Description : |
Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1). Expression of MIC-1 mRNA in monocytoid cells is up-regulated by a variety of stimuli associated with activation, including interleukin 1β, tumor necrosis factor α (TNF-α), interleukin 2, and macrophage colony-stimulating factor but not interferon γ, or lipopolysaccharide (LPS). It is highly expressed in cardiomyocytes, adipocytes, macrophages, endothelial cells, and vascular smooth muscle cells in normal and pathological condition. GDF-15 increases during tissue injury and inflammatory states and is associated with cardiometabolic risk. Increased GDF-15 levels are associated with cardiovascular diseases such as hypertrophy, heart failure, atherosclerosis, endothelial dysfunction, obesity, insulin resistance, diabetes, and chronic kidney diseases in diabetes. Increased GDF-15 level is linked with the progression and prognosis of the disease condition. |
| Form : |
Lyophilized from 0.22 μm filtered solution in 0.085%TFA 30%ACN with trehalose as protectant. |
| Bio-activity : |
Immobilized Mouse GDF-15, His Tag at 1 μg/mL (100 μL/well) can bind Human GFR alpha-like, Fc Tag with a linear range of 0.3-10 ng/mL. |
| Molecular Mass : |
The protein has a calculated MW of 14.6 kDa. The protein migrates as 16-17 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE). |
| Endotoxin : |
Less than 1.0 EU/μg by the LAL method/rFC method. |
| Purity : |
>90% as determined by SDS-PAGE. |
| Storage : |
This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
