Active Recombinant Mouse GPC3 protein, Fc-tagged

Cat.No. : GPC3-1516M
Product Overview : Recombinant Mouse GPC3 protein(Gln25-Met557), fused with Fc tag, was expressed in HEK293.
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Source : HEK293
Species : Mouse
Tag : Fc
Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Bio-activity : Immobilized Human FGF basic, premium grade at 2 μg/mL (100 μL/well) can bind Mouse Glypican 3, Fc Tag with a linear range of 1-10 ng/mL.
Molecular Mass : This protein carries a human IgG1 Fc tag at the C-terminus. This protein contains a furin-like convertase cleavage site, 354-RQYR-357, and will be partially processed into N and C-terminal fragment with calculated MW of 38.0 kDa and 49.1 kDa respectively. The protein migrates as 40 kDa, 60 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Proteinlength : Gln25-Met557
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Gpc3 glypican 3 [ Mus musculus ]
Official Symbol : GPC3
Synonyms : GPC3; glypican 3; glypican-3; OCI-5;
Gene ID : 14734
mRNA Refseq : NM_016697
Protein Refseq : NP_057906

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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08/12/2020

    The GPC3 protein offered by the manufacturer is of exceptional quality, making it an ideal choice to fulfill my experimental needs.

    09/18/2019

      The technical support provided by the manufacturer is invaluable in overcoming experimental hurdles and optimizing the utilization of GPC3 protein.

      04/10/2019

        Another crucial aspect where the manufacturer excels is their efficient supply chain management.

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