Active Recombinant Mouse Growth Differentiation Factor 3

• Cat.No.: Gdf3-718M
• Product Overview: Recombinant Mouse Gdf3protein was expressed in E. coli. Ala253-Gly366.

Specification

• Cat. No.: Gdf3-718M
• Description: Gdf3 is a TGFβsuperfamily member belonging to the growth/differentiation factor family.Gdf3 is expressed in undifferentiated embryonic stem (ES) cells, adiposetissue and the brain. In ES cells, it maintains pluripotency and influencesearly cell fate decisions. For example, frog embryos injected with Gdf3develop a secondary dorsal axis and deletion of mouse Gdf3 can producedefects in the anterior visceral endoderm of the pregastrulation embryo.
• Source: E. coli-derived.
• Form: Lyophilized from a0.2 μm filtered solution in Acetonitrile and TFA.
• N-terminalSequence: Ala253
• Molecular Weight: 13.1 kDa
• Activity: Measuredby its ability to bind with Activin RIB/Fc Chimera in the presence ofrmCripto in functional ELISA. Chen, et al. (2005) Development 133:319.Immobilized rmGdf3 at 1 μg/mL (100 μL/well) can bind rhActivin RIB/Fc Chimerawith an apparent KD < 100 nM.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in sterile 4 mM HCI
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: Gdf3 growth differentiationfactor 3 [ Mus musculus ]
• Official Symbol: Gdf3
• Synonyms: Gdf3; growthdifferentiation factor 3; Vgr2; Gdf-3; Vgr-2; ecat9; C78318; MGC123990;MGC123991; growth/differentiation factor 3; VG-1-related protein 2
• Gene ID: 14562
• mRNA Refseq: NM_008108
• Protein Refseq: NP_032134
• UniProt ID: Q07104
• Chromosome Location: 6 F1; 6 60.6 cM
• Function: cytokine activity;growth factor activity; protein binding