Active Recombinant Mouse Il12a Protein (Arg23-Ala215), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Il12a-47M
• Product Overview: Recombinant Mouse Il12a Protein (Arg23-Ala215) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Interleukin 12 (IL-12) is a pro-inflammatory cytokine with a molecular weight of 70 kDa composed of two subunits, IL-12 p35 (35 kDa) and IL-12 p40 (40 kDa), and IL-12 p35 as a p35-p35 homo-dimer with 57 kDa molecular weight and a p35 monomer of 27 kDa molecular weight. The p35 molecule is unique to IL-12, while p40 is shared by both IL-12 and IL-23. IL-12 promotes Th1 T cell responses, while IL-23 promotes Th17 T cell responses.
• Source: E. coli
• Species: Mouse
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in T-cell enriched PBMC. The ED50 for this effect is < 0.2 ng/mL.
• Molecular Mass: The protein has a calculated MW of 22.65 kDa.
  The protein migrates about 25 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Arg23-Ala215
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Il12a interleukin 12a [ Mus musculus (house mouse) ]
• Official Symbol: Il12a
• Synonyms: IL12A; interleukin 12a; interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; interleukin 12a p35 subunit; cytotoxic lymphocyte maturation factor 35 kDa subunit; p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232
• Gene ID: 16159
• mRNA Refseq: NM_001159424
• Protein Refseq: NP_001152896
• UniProt ID: P43431