Active Recombinant Mouse Il28b Protein (Asp20-Val193), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Il28b-153M
• Product Overview: Recombinant Mouse Il28b Protein (Asp20-Val193) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Interleukin 28B (IL-28B) predicts a molecular mass of 19.7 kDa. IL-28 comes in two isoforms, IL-28A and IL-28B, and plays a role in immune defense against viruses, including the induction of an "antiviral state" by turning on Mx proteins, 2',5'-oligoadenylate synthetase as well as ISGF3G.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is < 20 ng/mL.
• Molecular Mass: The protein has a calculated MW of 20.56 kDa.
  The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Asp20-Val193
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 2 weeks under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Il28b interleukin 28B [ Mus musculus (house mouse) ]
• Official Symbol: Il28b
• Synonyms: IL28B; interleukin 28B; interleukin-28B; IFN-lambda-3; interleukin 28; interferon alpha; interferon lambda-3; Il28; IFL-1; IL-28B; INF-alpha; INF-lambda
• Gene ID: 338374
• mRNA Refseq: NM_177396
• Protein Refseq: NP_796370
• UniProt ID: Q8CGK6

Reviews

07/24/2022

Activity retention time is very long and can be maintained for a long time.

02/04/2022

High cost performance and low preparation cost.

01/24/2022

It has a wide range of applications and can be used in various biological experiments.

Q&As

IL28B has made a breakthrough in treating what diseases?

IL28B has achieved remarkable results in the treatment of hepatitis C. The study found that patients with a specific genotype, which is related to IL28B, were better able to benefit from treatment with the hepatitis drug interferon. Therefore, the combination of IL28B with other drugs can improve the efficacy.

What is the mechanism of action of IL28B?

IL28B can regulate the immune response of cells and promote the occurrence of insulin-dependent cytotoxicity mediated by NK cells, thus playing an antiviral role.

IL28B purification ofs have?

The purification methods of IL28B include ion exchange, gel filtration, affinity chromatography, countercurrent chromatography, etc.

How many therapeutic doses of IL28B are usually used?

The dose of treatment with IL28B varies depending on the patient. When treating hepatitis C, the dose is usually given once a week, 500ug or another dose.

What are the adverse reactions of IL28B?

The adverse reactions of IL28B include fever, headache, fatigue, muscle aches, nausea, diarrhea and other symptoms, and in severe cases, allergic reactions and liver damage.

Does IL28B affect the function of other organs in the body?

IL28B will have a certain impact on the function of other organs of the body, such as the liver, kidneys, etc., but the use of normal doses in clinical practice is generally safe.