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Active Recombinant Mouse Il33 Protein, His-Tagged

Cat.No. : Il33-01M
Product Overview : Recombinant mouse Il33 Protein, His-tagged was expressed in E. coli cell
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Description : Interleukin 33 (IL-33) is a member of the IL-1 family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL-4). IL33 is a ligand for ST2 (IL1RL1), an IL-1 family receptor that is highly expressed on Th2 cells, mast cells and group 2 innate lymphocytes. It is expressed by a wide variety of cell types, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells.
Source : Escherichia coli
Species : Mouse
Tag : His
Form : Lyophilized powder
AA Sequence : MSIQGTSLLTQSPASLSTYNDQSVS FVLENGCYVINVDDSGKDQEQDQVL LRYYESPCPASQSGDGVDGKKLMVN MSPIKDTDIWLHAND
KDYSVELQRGDVSPPEQAFFVLHKK SSDFVSFECKNLPGTYIGVKDNQLA LVEEKDESCNNIMFKLSKI with polyhistidine tag at the C-terminus
Endotoxin : <0.1 EU per 1 μg of the protein by the LAL method.
Bio-activity : Measure by its ability to induce proliferation in D10.G4.1 cells. The ED50 for this effect is <40 pg/mL. The specific activity of recombinant mouse IL-33 is > 2 x 10^7 IU/mg.
Purity : >98% as determined by SDS-PAGE. Ni-NTA chromatography
Storage Buffer : The protein was lyophilized from a solution containing 1X PBS, pH 7.4.
Reconstitution : It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Storage : Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Notes : Please use within one month after protein reconstitution.
Gene Name : Il33 interleukin 33 [ Mus musculus (house mouse) ]
Official Symbol : Il33
Synonyms : Il-33; Il1f11; NF-HEV; 9230117N10Rik
Gene ID : 77125
mRNA Refseq : NM_001164724.2
Protein Refseq : NP_001158196.1
UniProt ID : Q8BVZ5

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Which immune cells can recombinant IL33 be used to detect? 05/28/2022

It can be used to detect immune cells such as macrophages and dendritic cells.

In what research fields can recombinant IL33 be applied? 09/24/2020

This protein applied in immunology, drug research, life science and other fields.

What is the action mechanism of IL33? 08/09/2020

Regulates the activity of immune cells by binding to receptors to stimulate the production of inflammatory factors such as interferon and TNF-α.

Besides treating asthma and hay fever, what other diseases can be treated with IL33? 07/28/2019

IL33 can also be used to treat a variety of inflammatory diseases, such as inflammatory bowel disease, psoriasis and so on.

How to prepare IL33? 03/25/2019

The preparation of IL33 requires first cloning the IL33 gene into the expression vector, and then the expression vector is expressed and purified in the cell culture medium.

How long does it take to treat asthma with IL33? 01/10/2019

Treating asthma with recombinant IL33 takes several weeks to several months to be effective, depending on the severity of the condition and the individual patient.

Customer Reviews (3)

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Reviews
10/16/2021

    Good solubility and is easy to operate and purify.

    05/03/2021

      Effectively mimics the function of the target protein in vitro.

      03/18/2020

        IL33 has a wide range of applications in the field of scientific research and is suitable for a variety of experimental design and research purposes.

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