Active Recombinant Mouse Il34 Protein, His-tagged

• Cat.No.: Il34-3522M
• Product Overview: Purified recombinant protein of Mouse interleukin 34 (Il34), transcript variant 1 with a C-His tag was expressed in CHO.

Specification

• Species: Mouse
• Source: CHO
• Tag: His
• Protein Length: Met1- Pro235
• Description: Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.
• Bio-activity: The ED50 is 20 - 30 ng/mL, corresponding to a specific activity of 3.3 - 5 × 10^4 units/mg, determined by M-NFS-60 cell proliferation induction in a dose dependent manner.
• Molecular Mass: 26 kDa
• Endotoxin: Less than 0.01 ng/μg protein as determined by the LAL method
• Purity: > 98%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 μg/mL in specified buffer
• Storage Buffer: 10 mM NaH2PO4, 0.5 M NaCl, pH 7.4

Gene Information

• Gene Name: Il34 interleukin 34 [ Mus musculus (house mouse) ]
• Official Symbol: Il34
• Synonyms: IL34; interleukin 34; interleukin-34; IL-34; AI593503; 2010004A03Rik
• Gene ID: 76527
• mRNA Refseq: NM_001135100
• Protein Refseq: NP_001128572
• UniProt ID: Q8R1R4