Active Recombinant Mouse Il15 protein, His-tagged

• Cat.No.: Il15-739M
• Product Overview: Recombinant Mouse Il15 protein(Asn49-Ser162), fused with C-terminal His tag, was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: 49-162 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1. Measured in a cell proliferation assay using HT-2 mouse T cells. The ED50 for this effect is 15-58 ng/mL, corresponding to a specific activity of 1.72×10^4-6.67×10^4 units/mg. 2. Measured in a cell proliferation assay using CTLL‑2 mouse T cells. The ED50 for this effect is 8.1‑32.4ng/mL,corresponding to a specific activity of 3.09×10^4~1.23×10^5 units/mg.
• Molecular Mass: 14.09 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Il15 interleukin 15 [ Mus musculus ]
• Official Symbol: Il15
• Synonyms: IL15; interleukin 15; interleukin-15; IL-15; AI503618
• Gene ID: 16168
• mRNA Refseq: NM_001254747
• Protein Refseq: NP_001241676