Active Recombinant Mouse Interleukin 2 Receptor, Alpha Chain, His-tagged

• Cat.No.: Il2ra-5654M
• Product Overview: RecombinantMouse Il2ra protein was expressed in Murine myeloma cellline.Glu22-Lys236, with a C-terminal 10-His tag.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• ProteinLength: 22-236 a.a.
• Description: Il2ra isa 55 kDa type I membrane glycoprotein that belongs to the family of cytokinereceptors that utilize the common gamma chain subunit. Il2ra is primarilyexpressed on activated T cells and on regulatory T cells (Treg). The mouse Il2racDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signalpeptide, a 215 aa extracellular domain (ECD) with two Sushi domains, a 21 aatransmembrane segment, and an 11 aa cytoplasmic domain. Within the ECD, mouseIl2ra shares 81% and 58% aa sequence identity with rat and human Il2ra,respectively.
• Form: Lyophilizedfrom a 0.2 μm filtered solution in PBS.
• N-terminalSequence: Glu22
• MolecularWeight: 26 kDa
• Activity: Measuredby its ability to inhibit the IL-2-dependent proliferation of MO7e humanmegakaryocytic leukemic cells. The ED50 for this effect is typically 0.1-0.4μg/mL in the presence of 30 ng/mL of rhIL-2.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 40-50kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mLin sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Il2ra interleukin 2 receptor,alpha chain [ Mus musculus ]
• Official Symbol: Il2ra
• Synonyms: Il2ra; interleukin 2receptor, alpha chain; CD25; Il2r; Ly-43; interleukin-2receptor subunit alpha; IL2-RA; IL-2-RA; p55 chain; IL-2R alpha chain; IL-2Rsubunit alpha; IL-2 receptor subunit alpha; Interleukin-2 receptor alpha subunit
• Gene ID: 16184
• mRNA Refseq: NM_008367
• Protein Refseq: NP_032393
• UniProt ID: Q544I2
• Chromosome Location: 2
• Pathway: Cytokine Signalingin Immune system; Cytokine-cytokine receptor interaction; Endocytosis; HTLV-Iinfection; Hematopoietic cell lineage; IL-2 Signaling Pathway; Immune System;Inflammatory Response Pathway; Interleukin receptor SHC signaling; Interleukin-2signaling; Interleukin-3, 5 and GM-CSF signaling; Jak-STAT signaling pathway;Measles; Signaling by Interleukins
• Function: drug binding; interleukin-2binding; interleukin-2 receptor activity; protein binding; receptor activity