Active Recombinant Mouse ITGAV&ITGB8 protein, His-tagged

• Cat.No.: ITGAV&ITGB8-0168M
• Product Overview: Recombinant Mouse ITGAV&ITGB8 protein(Phe31-Val988(ITGAV)&Gly22-Leu681(ITGB8)), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag with a linear range of 1-10 ng/mL.Immobilized Human Fibronectin at 2 μg/mL (100 μL/well) can bind Mouse ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.156-5 μg/mL.Immobilized Human Vitronectin at 2 μg/mL (100 μL/well) can bind Mouse ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.156-5 μg/mL.
• Molecular Mass: The non-reducing (NR) protein migrates as 130-150 kDa (ITGAV) and 80-90 kDa (ITGB8) respectively due to glycosylation.
• Proteinlength: Phe31-Val988(ITGAV)&Gly22-Leu681(ITGB8)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Itgav integrin alpha V [ Mus musculus ]
• Official Symbol: ITGAV
• Synonyms: ITGAV; integrin alpha V; integrin alpha-V; alphav-integrin; vitronectin receptor subunit alpha; vitronectin receptor alpha polypeptide (VNRA); CD51; 1110004F14Rik; 2610028E01Rik; D430040G12Rik
• Gene ID: 16410
• mRNA Refseq: NM_008402
• Protein Refseq: NP_032428