Species : |
Mouse |
Source : |
Hordeum Vulgare |
Tag : |
Non |
Description : |
LIF (leukemia inhibitory factor, myeloid leukemia inhibitory factor) a pleiotropic lymphoid factor was initially described as a factor that inhibits the proliferation of myeloid leukemia cells and induces their differentiation into macrophages. Other activities of LIF include inhibition of adipogenesis, cholinergic neuron differentiation and bone metabolism. |
Predicted N Terminal : |
His |
Form : |
PBS, pH 7.2, sterile filtered. |
Bio-activity : |
The bioactivity of murine LIF was determined by its ability to induce differentiation of murine M1 myeloid leukemia cells. The ED50 value for this effect is typically< 1="" ng/ml,="" corresponding="" to="" a="" specific="" activity="" of="">5 x 10e7 U/mg (where 50 U is defined as the amount of mLIF required to induce differentiation of 50% of M1 cells). The activity is comparable to mLIF from other commercial sources. |
Molecular Mass : |
Recombinant mouse LIF contains 188 amino acids and a 16 a.a. Histidine-based tag for a total length of 204 a.a. and has a predicted molecular mass of 22.8 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 28-30 kDa in SDS-PAGE. |
Endotoxin : |
Endotoxin level is less than 0.05EU/μg as measured by turbidimetric kinetic assay. |
Purity : |
>95% by SDS-PAGE gel analysis. |
Storage : |
The lyophilized protein is stable at room temperature for few weeks. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |