| Species : |
Mouse |
| Source : |
CHO |
| Tag : |
Non |
| Protein Length : |
279-390 aa |
| Description : |
Transforming Growth Factor beta 1 (TGF β 1/TGFB1 Protein) is a multifunctional cytokine synthesized by almost all cells. It demonstrates significant amino acid sequence stability across diverse species, underscoring its conserved function throughout evolution. TGF β 1/TGFB1 Protein is secreted as an inactive peptide, forming a 'latent complex' composed of a mature TGFB1 dimer non-covalently bound to its latency-associated peptide (LAP), which subsequently links to latent TGFB-binding proteins (LTBPs). The activated TGF β 1/TGFB1 Protein binds to ubiquitously expressed cell-surface transmembrane serine/threonine kinase receptors, specifically the type I (TGFBRI) and type II (TGFBRII) receptors, with a high binding ability to TGFbRII. This receptor engagement regulates various critical cellular processes including proliferation, growth, differentiation, and cell movement. Moreover, TGF β 1/TGFB1 Protein exerts immunomodulatory and profibrogenic effects, acting both locally and systemically, and plays a pivotal role in development, fibrosis, and cancer. |
| Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM HAC, pH3.0 with trehalose as protectant. |
| Bio-activity : |
Immobilized Mouse TGF-Beta 1 Protein, Tag Free at 0.2 μg/mL (100 μL/well) can bind Biotinylated Mouse TGF-beta RII, His, Avitag with a linear range of 0.1-3 ng/mL. |
| Molecular Mass : |
The protein has a calculated MW of 12.8 kDa. The protein migrates as 13-14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE). |
| Purity : |
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC. |
| Storage : |
This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
