Active Recombinant Human TGFB1 protein, His-tagged

Cat.No. : TGFB1-429H
Product Overview : Active Recombinant Human TGFB1 protein(P01137)(279~390 aa), fused with N-terminal His tag, was expressed in E.coli.
Availability September 29, 2025
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Species : Human
Source : E.coli
Tag : His
Protein Length : 279-390 aa
Form : Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Bio-activity : TGF-β1 (Transforming growth factor beta 1) is a multifunctional set of peptides that controls proliferation, differentiation, and other functions in many cell types. TGF beta 1 has been shown to interact with TGF beta receptor 1, Decorin, LTBP1 and so on. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant human TGF-β1 with recombinant human LTBP1. Briefly, TGF-β1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to LTBP1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-LTBP1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TGF-β1 with LTBP1 was shown and this effect was in a dose dependent manner.
Molecular Mass : 14.1kDa
Purity : > 97% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]
Official Symbol TGFB1
Synonyms TGFB1; transforming growth factor, beta 1; DPD1, TGFB; transforming growth factor beta-1; Camurati Engelmann disease; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide; LAP; DPD1; TGFB;
Gene ID 7040
mRNA Refseq NM_000660
Protein Refseq NP_000651
MIM 190180
UniProt ID P01137

Not For Human Consumption!

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