Active Recombinant Human TGFB1 protein, His-tagged
Cat.No. : | TGFB1-429H |
Product Overview : | Active Recombinant Human TGFB1 protein(P01137)(279~390 aa), fused with N-terminal His tag, was expressed in E.coli. |
Availability | September 29, 2025 |
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Species : | Human |
Source : | E.coli |
Tag : | His |
Protein Length : | 279-390 aa |
Form : | Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
Bio-activity : | TGF-β1 (Transforming growth factor beta 1) is a multifunctional set of peptides that controls proliferation, differentiation, and other functions in many cell types. TGF beta 1 has been shown to interact with TGF beta receptor 1, Decorin, LTBP1 and so on. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant human TGF-β1 with recombinant human LTBP1. Briefly, TGF-β1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to LTBP1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-LTBP1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TGF-β1 with LTBP1 was shown and this effect was in a dose dependent manner. |
Molecular Mass : | 14.1kDa |
Purity : | > 97% as determined by SDS-PAGE. |
Storage : | Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
Reconstitution : | Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name | TGFB1 transforming growth factor, beta 1 [ Homo sapiens ] |
Official Symbol | TGFB1 |
Synonyms | TGFB1; transforming growth factor, beta 1; DPD1, TGFB; transforming growth factor beta-1; Camurati Engelmann disease; CED; TGFbeta; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide; LAP; DPD1; TGFB; |
Gene ID | 7040 |
mRNA Refseq | NM_000660 |
Protein Refseq | NP_000651 |
MIM | 190180 |
UniProt ID | P01137 |
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Not For Human Consumption!
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