Active Recombinant Mouse Tnfrsf10b protein, His&hFc-tagged

• Cat.No.: Tnfrsf10b-3321M
• Product Overview: Recombinant Mouse Tnfrsf10b protein(NP_064671.2)(Met1-Ser177), fused with His&hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met1-Ser177
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Immobilized human TNFSF10 (Cat:10409-HNAE) at 10 μg/ml (100 μl/well) can bind mouse TNFRSF10B-Fch, The EC50 of mouse TNFRSF10B-Fch is 0.07-0.17 μg/ml.
• Molecular Mass: The recombinant mouse TNFRSF10B/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 373 amino acids and has a calculated molecular mass of 41.8 kDa. As a result of glycosylation, the apparent molecular mass of the TNFRSF10B/Fc monomer is approximately 50-55 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Tnfrsf10b tumor necrosis factor receptor superfamily, member 10b [ Mus musculus ]
• Official Symbol: Tnfrsf10b
• Synonyms: TNFRSF10B; tumor necrosis factor receptor superfamily, member 10b; tumor necrosis factor receptor superfamily member 10B; death receptor 5; KILLER/DR5 TRAIL death-inducing receptor; MK; DR5; Ly98; KILLER; TRICKB; TRAILR2; TRICK2A; TRICK2B
• Gene ID: 21933
• mRNA Refseq: NM_020275
• Protein Refseq: NP_064671