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Active Recombinant Mouse Vcam1, His-tagged

Cat.No. : Vcam1-4013M
Product Overview : A DNA sequence encoding the extracellular domain ( Met 1 - Glu 698 ) of mouse VCAM1 ( NP_035823.3 ) was fused with the a polyhistidine tag at the C-terminus.
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Cat. No. : Vcam1-4013M
Description : Mouse vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two isoforms of mouse VCAM1 have been identified, referred to as single-pass type I transmembrane (short) isoform 1 containing seven C2-type I g-like domains and GPI-anchored isoform 2. Mouse VCAM1 is expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
Source : Mouse
Host : Human Cells
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
Purity : > 97 % as determined by SDS-PAGE
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells / well are added to mouse VCAM1 coated plates ( 10 μg/mL with 100 μL/well ) , approximately 70% - 80% cells will adhere after 1 hour incubation at RT
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Stability : Samples are stable for up to twelve months from date of receipt at -70μ
Predicted N terminal : Phe 25
Molecular Mass : The secreted recombinant mouse VCAM1 consists of 685 amino acids and has a predicted molecular mass of 75.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmVCAM1 is approximately 90-100 kDa due to glycosylation.
Storage : Store it under sterile conditions at -70μ. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Gene Name : Vcam1 vascular cell adhesion molecule 1 [ Mus musculus ]
Official Symbol : Vcam1
Synonyms : Vcam1; vascular cell adhesion molecule 1; CD106; Vcam-1
Gene ID : 22329
mRNA Refseq : NM_011693
Protein Refseq : NP_035823
UniProt ID : P29533
Chromosome Location : 3 G1; 3 50.8 cM
Pathway : Cell adhesion molecules (CAMs); Leukocyte transendothelial migration
Function : protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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