| Species : | Pig | 
                                
                                    | Source : | E.coli | 
                                
                                    | Tag : | His | 
                                
                                    | Protein Length : | 53-172 aa | 
                                
                                    | Form : | Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. | 
                                
                                    | Bio-activity : | TNF-β, a member of the tumor necrosis factor family, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells . The biological effects of TNF-β are very similar to TNF-α,due to the similarity of molecular structure and the receptors.As reported, TNF-α could inhibit the proliferation and induce apoptosis of A549 cells,therefore, A549 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well with 5% serum standard DMEM including various concentrations of recombinant pig TNF-β. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2 hours at 37°C. Proliferation of A549 cells after incubation with TNF-β for 48h observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant pig TNF-β for 48h. It was obvious that TNF-β significantly inhibit cell viability of A549 cells. The ED50 is 3.5μg/ml. | 
                                
                                    | Molecular Mass : | 16.7kDa | 
                                
                                    | Endotoxin : | <1.0EU per 1μg (determined by the LAL method). | 
                                
                                    | Purity : | > 90% as determined by SDS-PAGE. | 
                                
                                    | Storage : | Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. | 
                                
                                    | Reconstitution : | Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |