Active Recombinant Porcine IL18 Protein, His-tagged

• Cat.No.: IL18-946P
• Product Overview: Recombinant Porcine IL18 (Accession # O19073) Tyr36-Asn192, fused with a C-terminal 6-His tag, was produced in E. coli.

Specification

• Species: Porcine
• Source: E.coli
• Tag: His
• Protein Length: Tyr36-Asn192
• Predicted N Terminal: Tyr36
• Form: Lyophilized from a 0.2 µm filtered solution in PBS and DTT with Trehalose and with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha.The ED50 for this effect is typically 10 - 40 ng/mL in the presence of 10 ng/mL rhTNF-alpha.
• Molecular Mass: Recombinant Porcine IL18, His-tagged has a calculated MW of 18.0 kDa.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CHST10 carbohydrate sulfotransferase 10 [ Sus scrofa (pig) ]
• Official Symbol: CHST10
• Synonyms: CHST10; carbohydrate sulfotransferase 10
• Gene ID: 1.0052e+008
• mRNA Refseq: XM_003124889.4
• Protein Refseq: XP_003124937.3
• Chromosome Location: chromosome: 3

Q&As

What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy)

IL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.