Active Recombinant Rabbit PDCD1 protein, His-tagged

• Cat.No.: PDCD1-371R
• Product Overview: Recombinant Rabbit PDCD1 protein(Leu 25 - Gln 167), fused with His tag, was expressed in HEK293.

Specification

• Species: Rabbit
• Source: HEK293
• Tag: His
• Protein Length: Leu 25 - Gln 167
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Rabbit PD-L1, His Tag immobilized on CM5 Chip can bind Rabbit PD-1, His Tag with an affinity constant of 3.87 μM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 17.9 kDa. The protein migrates as 33-36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.