Active Recombinant Rabbit TNF, Met-tagged

• Cat.No.: TNF-668R
• Product Overview: Recombinant Rabbit TNF (Accession # NP_001075732) Leu79-Leu234, fused with an N-terminal Met, was produced in E. coli-derived.

Specification

• Species: Rabbit
• Source: E.coli
• Protein Length: 79-234 a.a.
• Predicted N Terminal: Met - Leu79
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.The ED50 for this effect is typically 0.01-0.04 ng/mL.
• Molecular Mass: Recombinant Rabbit TNF, Met-tagged has a calculated MW of 17.4 kDa. In SDS-PAGE migrates as 19 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: TNF tumor necrosis factor [ Oryctolagus cuniculus ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; TNF-a; TNF-alpha; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2; TNFA; TNFSF2
• Gene ID: 100009088
• mRNA Refseq: NM_001082263
• Protein Refseq: NP_001075732