Active Recombinant Rat Camkk1, GST-tagged

• Cat.No.: Camkk1-106R
• Product Overview: Recombinant full-length rat CAMKK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: CAMKK1 or CAMKKalpha is a Ca(2+)/calmodulin-dependent protein kinase that activates CAM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively. Recent studies have shown that the activity of CAMKK1 is decreased upon phosphorylation by cAMP-dependent protein kinase (PKA) The CAMKKalpha has been identified in intact cells as AMPKKs, predicting a significant role for this kinase in regulating AMPK activity in vivo. It has been shown that 2-deoxyglucose- and ionomycin-stimulated AMPK activity is substantially reduced in HeLa cells transfected with small interfering RNAs specific for CAMKKalpha.
• Source: Sf9 insect cells using baculovirus
• Species: Rat
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: The specific activity of CAMKK1 was determined to be 9 nmol /min/mg
• Molecular Mass: ~88 kDa
• Purity: >85% by densitometry
• Applications: Kinase Assay, Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: Camkk1 calcium/calmodulin-dependent protein kinase kinase 1, alpha [ Rattus norvegicus ]
• Official Symbol: Camkk1
• Synonyms: CAMKK1; calcium/calmodulin-dependent protein kinase kinase 1, alpha; calcium/calmodulin-dependent protein kinase kinase 1; caMKK 1; caM-KK 1; alpha CaMKK; caMKK alpha; caM-KK alpha; caM-kinase kinase 1; caM-kinase IV kinase; caM-kinase kinase alpha; calcium/calmodulin-dependent protein kinase kinase alpha
• Gene ID: 60341
• mRNA Refseq: NM_031662
• Protein Refseq: NP_113850
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem
• Function: ATP binding; calmodulin binding; calmodulin-dependent protein kinase activity; calmodulin-dependent protein kinase activity; nucleotide binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity