Active Recombinant Rat Cd274 protein, hFc-tagged

• Cat.No.: Cd274-561R
• Product Overview: Recombinant Rat Cd274 Protein (NP_001178883.1, 1-238aa), was expressed in HEK293 with C-terminal hFc tag.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: 1-238 aa
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized rat CD274-Fc at 10 μg/ml (100 μl/well) can bind biotinylated rat PDCD1-Fc, The EC50 of biotinylated rat PDCD1-Fc is 0.14-0.34 μg/ml.
• Molecular Mass: The recombinant rat CD274/Fc is a disulfide-linked homodimer. The reduced monomer comprises 459 amino acids and has a predicted molecular mass of 51.8 kDa. The apparent molecular mass of the protein is approximately 73.2 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd274 CD274 molecule [ Rattus norvegicus ]
• Official Symbol: Cd274
• Synonyms: CD274; CD274 molecule; programmed cell death 1 ligand 1; Pdcd1lg1; RGD1566211
• Gene ID: 499342
• mRNA Refseq: NM_001191954
• Protein Refseq: NP_001178883