Active Recombinant Human CD274 protein, hFc-tagged, Low Endotoxin

• Cat.No.: CD274-358H
• Product Overview: Recombinant Human CD274 protein(Q9NZQ7-1)(Phe19-Arg238), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 19-238 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 8% trehalose (pH 7.4).
• Bio-activity: Immobilized Human PD-1, mFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.12 μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 52 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CD274 CD274 molecule [ Homo sapiens ]
• Official Symbol: CD274
• Synonyms: CD274; CD274 molecule; CD274 antigen, PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296
• Gene ID: 29126
• mRNA Refseq: NM_014143
• Protein Refseq: NP_054862
• MIM: 605402
• UniProt ID: Q9NZQ7