Species : |
Rat |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
Met1-Ala229 |
Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : |
Measured by its binding ability in a functional ELISA.1. Immobilized rat EFNB2-His at 10 μg/mL (100 μl/well) can bind mouse EPHB4-Fc, The EC50 of mouse EPHB4-Fc is 19.0-44.5 ng/mL. 2. Immobilized rat EFNB2-His at 10 μg/mL (100 μl/well) can bind human EphB4-Fc, The EC50 of human EphB4-Fc is 10.0-23.5 ng/mL. |
Molecular Mass : |
The recombinant rat EFNB2 comprises 212 amino acids and has a predicted molecular mass of 23.5 kDa. The apparent molecular mass of the protein is approximately 33-39 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
Purity : |
> 99 % as determined by SDS-PAGE |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |