Active Recombinant Rat IL12 protein, His-tagged

• Cat.No.: IL12-27R
• Product Overview: Recombinant Rat Interleukin-12 is expressed in CHO cells and the target gene encoding Met23-Ser335(p40)&Arg23-Ser215(p35) is expressed with a 6His tag at the C-terminus.

Specification

• Species: Rat
• Source: Mammalian Cells
• Tag: His
• Protein Length: 23-335;23-215 a.a.
• Description: Interleukin-12 (IL-12), also known as NKSF, TCMF, CLMF and TSF, is a heterodimeric cytokine composed of p35 and p40 subunits. It is produced by monocytes, macrophages, B cells and dendritic cells in response to bacterial lipopolysaccharides and intracellular pathogens. IL-12 signals through the IL-12 receptor complex, which is comprised of IL-12 Rβ1 and IL-12 Rβ2. IL-12 induces the proliferation and activation of hematopoietic stem cells, natural killer cells and T- cells. It is indispensible during the development of Th1 cells, leading to the production of IFN-gamma and IL-2.
• Form: Lyophilized after extensive dialysis against PBS.
• Bio-activity: ED50< 0.1ng/ml, measured in a cell proliferation assay using 2D6 cells.
• Molecular Mass: 26-28kDa (p35) and 42-45 kDa (p40), observed by reducing SDS-PAGE.
• AA Sequence: p35:RVIPVSGPAKCLNQSQNLLKTTDDMVRTAREKLKHYSCTAGDIDHEDITRDKTSTLEACLPLELHKNESCLATKETSSIIRGSCLPPQKTSLMMTLCLGSIYEDLKMYQSEFQAINAALQSHNHQQITLDRNMLMAIDELMRSLNHSGETLHQKAPMGEADPYRVKMKLCILLHAFSTRVMTINRVMNYLSSSHHHHHH
  p40: MWELEKDVYV VEVDWRPDAP GETVTLTCDS PEEDDITWTS DQRRGVIGSG KTLTITVREF LDAGQYTCHR GGETLSHSHL LLHKKENGIW STEILKNFKNKTFLKCEAPN YSGRFTCSWLVHRNTDLKFNIKSSSSSPESRAVTCGRASLSAEKVTLNQRDYEKYSVACQEDVTCPTAEETLPIELVVEAQQQNKYENYSTSFFIRDIIKPDPPKNLQVKPLKNSQVEVS WEYPDSWSTPHSYFSLKFFVRIQRKKEKTKETEEECNQKGAFLVEKTSAEVQCKGANICVQAQDRYYNSSCSKWTCVPCRGRS
• Endotoxin: <0.2 EU/μg, determined by LAL method.
• Purity: > 95% as analyzed by SDS-PAGE.
• Storage: Lyophilized recombinant rat Interleukin-12 (IL-12), His remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rat Interleukin-12 (IL-12), His should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/ml.

Gene Information

• Gene Name: interleukin 12A [ Rattus norvegicus ]
• Official Symbol: IL12A
• Synonyms: Interleukin-12; IL-12; IL12A
• Gene ID: 84405
• mRNA Refseq: NM_053390.1
• Protein Refseq: NP_445842.1
• UniProt ID: Q9R103