Active Recombinant Rat IL12 protein, His-tagged
|Product Overview :||Recombinant Rat Interleukin-12 is expressed in CHO cells and the target gene encoding Met23-Ser335(p40)&Arg23-Ser215(p35) is expressed with a 6His tag at the C-terminus.|
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|Description :||Interleukin-12 (IL-12), also known as NKSF, TCMF, CLMF and TSF, is a heterodimeric cytokine composed of p35 and p40 subunits. It is produced by monocytes, macrophages, B cells and dendritic cells in response to bacterial lipopolysaccharides and intracellular pathogens. IL-12 signals through the IL-12 receptor complex, which is comprised of IL-12 Rβ1 and IL-12 Rβ2. IL-12 induces the proliferation and activation of hematopoietic stem cells, natural killer cells and T- cells. It is indispensible during the development of Th1 cells, leading to the production of IFN-gamma and IL-2.|
|Form :||Lyophilized after extensive dialysis against PBS.|
|Bio-activity :||ED50< 0.1ng/ml, measured in a cell proliferation assay using 2D6 cells.|
|Molecular Mass :||26-28kDa (p35) and 42-45 kDa (p40), observed by reducing SDS-PAGE.|
|AA Sequence :||p35:RVIPVSGPAKCLNQSQNLLKTTDDM VRTAREKLKHYSCTAGDIDHEDITR DKTSTLEACLPLELHKNESCLATKE TSSIIRGSCLPPQKTSLMMTLCLGS IYEDLKMYQSEFQAINAALQSHNHQ QITLDRNMLMAIDELMRSLNHSGET LHQKAPMGEADPYRVKMKLCILLHA FSTRVMTINRVMNYLSSSHHHHHH
p40: MWELEKDVYV VEVDWRPDAP GETVTLTCDS PEEDDITWTS DQRRGVIGSG KTLTITVREF LDAGQYTCHR GGETLSHSHL LLHKKENGIW STEILKNFKNKTFLKCEAPN YSGRFTCSWLVHRNTDLKFNIKSSS SSPESRAVTCGRASLSAEKVTLNQR DYEKYSVACQEDVTCPTAEETLPIE LVVEAQQQNKYENYSTSFFIRDIIK PDPPKNLQVKPLKNSQVEVS WEYPDSWSTPHSYFSLKFFVRIQRK KEKTKETEEECNQKGAFLVEKTSAE VQCKGANICVQAQDRYYNSSCSKWT CVPCRGRS
|Endotoxin :||<0.2 EU/μg, determined by LAL method.|
|Purity :||> 95% as analyzed by SDS-PAGE.|
|Storage :||Lyophilized recombinant rat Interleukin-12 (IL-12), His remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rat Interleukin-12 (IL-12), His should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade.|
|Reconstitution :||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Gene Name :||interleukin 12A [ Rattus norvegicus ]|
|Official Symbol :||IL12A|
|Synonyms :||Interleukin-12; IL-12; IL12A|
|Gene ID :||84405|
|mRNA Refseq :||NM_053390.1|
|Protein Refseq :||NP_445842.1|
|UniProt ID :||Q9R103|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Fluorescence microscopy tracks IL12 internalization and trafficking.
Phosphoproteomics and flow cytometry dissect IL12's signaling and crosstalk.
Flow cytometry and ELISA assess IL12's T cell subset polarization and cytokine profiles.
Reporter gene assays analyze IL12's promoter activity and response elements.
Super-resolution imaging details IL12's cellular distribution and dynamics.
3D cell culture models reveal IL12's effects on tissue microenvironments.
Co-localization studies and co-IP assays identify IL12's binding partners.
Techniques like co-immunoprecipitation reveal IL12 receptor complex assembly.
ChIP-seq helps map IL12-induced gene expression patterns and regulatory regions.
Transgenic models elucidate IL12's roles in immune response regulation.
Customer Reviews (3)Write a review
Effective in Th1 cell differentiation. -
High specificity in ELISA. -
Stable under storage. -
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