Active Recombinant Rat Il21r protein, His-tagged

• Cat.No.: Il21r-7452R
• Product Overview: Recombinant Rat Il21r protein(Q5EBB1)(Met1-Pro236), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met1-Pro236
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Immobilized rat IL21R-His at 10 μg/ml (100 μl/well) can bind biotinylated rat IL21-His, The EC50 of biotinylated rat IL21-His is 4.6-10.9 ng/ml.2. Immobilized Recombinant Rat IL-21 Protein at 2μg/mL (100μL/well) can bind Recombinant Rat IL-21R / Interleukin-21 Receptor Protein (His Tag), the EC50 is 80-320 ng/mL.
• Molecular Mass: The recombinant rat IL21R comprises 228 amino acids and predicts a molecular mass of 26.5 kDa. The apparent molecular mass of the recombinant protein is approximately 43-48 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il21r interleukin 21 receptor [ Rattus norvegicus ]
• Official Symbol: Il21r
• Synonyms: IL21R; interleukin 21 receptor; interleukin-21 receptor; MGC108921
• Gene ID: 308977
• mRNA Refseq: NM_001012469
• Protein Refseq: NP_001012487