Active Recombinant Rat SPARC Protein, His-tagged

• Cat.No.: SPARC-538R
• Product Overview: Recombinant Rat SPARC (Met1-Ile301) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ile301
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED50 for this effect is typically 1-5 μg/mL.
• Molecular Mass: The recombinant rat SPARC consists of 295 amino acids and predicts a molecular mass of 33.8 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Sparc secreted protein, acidic, cysteine-rich (osteonectin) [ Rattus norvegicus ]
• Official Symbol: SPARC
• Synonyms: SPARC; secreted protein, acidic, cysteine-rich (osteonectin); ON; BM-40; osteonectin; basement-membrane protein 40; secreted acidic cysteine rich glycoprotein; secreted protein acidic and rich in cysteine; Secreted acidic cystein-rich glycoprotein(osteonectin)
• Gene ID: 24791
• mRNA Refseq: NM_012656
• Protein Refseq: NP_036788