||The G-LISA series of Small G-Protein Activation Assays are ELISA based assays with which you can measure the GTP form of small G-proteins from lysates of cells or tissues and all in less than 3 h. The Cdc42 G-LISA Activation Assay measures the entire level of GTP-loaded Cdc42 protein in cell lysates. The level of activation is measured by reading at OD490nm. For a more detailed introduction on G-LISA assays and a listing of other available G-LISA kits, see our main G-LISA page. For a kit to measure RhoA activation please check webpage BK124, for Rac1 go to BK128 for Rac1,2,3 go to BK125, for RalA go to BK129.The Cdc42 G-LISA Activation Assay is very sensitive and has excellent accuracy for duplicate samples.
||Reagent-Quantity -Storage96 well Cdc42-GTP binding plate: 12 strips of 8 wells, Desiccated 4°C; Anti-Cdc42 antibody (mouse monoclonal): 1 tube, lyophilized, Desiccated 4°C; Secondary antibody-horseradish peroxidase conjugate (HRP): 2 tubes, lyophilized, Desiccated 4°C; Cdc42 control protein (constitutively active Cdc42): 2 tubes, lyophilized, Desiccated 4°C; CDCA Resuspension Buffer: 1 tube, liquid, 4°C; Cell Lysis Buffer: 1 bottle, lyophilized, Desiccated 4°C; Wash Buffer: 1 bottle, lyophilized, Desiccated 4°C; Antigen Presenting Buffer: 1 bottle, 25 ml, Room temperature; Antibody Dilution Buffer: 1 bottle, lyophilized, Desiccated 4°C; HRP Detection Reagent A: 1 tablet, silver pack, Desiccated 4°C; HRP Detection Reagent B: 1 tablet, gold pack, Desiccated 4°C; HRP Reagent Stop Solution: 2 bottles, 8 ml each, 4°C; Precision Red Advanced Protein Assay Reagent: (available as 500 ml size), 1 bottle, 100 ml, Room temperature; Protease Inhibitor Cocktail: 1 tube, lyophilized, Desiccated 4°C