COX Activity Assay Kit
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|Product Overview :||COX Activity Assay Kit measures the peroxidase activity of COX. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N",N"-tetramethyl-p-phenylenediamine (TMPD) at 590 nm. It can be used with both crude (cell lysates/tissue homogenates) and purified enzyme preparations. The kit includes isozymespecific inhibitors for distinguishing COX-2 activity from COX-1 activity.|
|Description :||Cyclooxygenase (COX, also called prostaglandin H Synthase or PGHS) is a bifunctional enzyme exhibiting both cyclooxygenase and peroxidase activities. The cyclooxygenase component converts arachidonic acid to a hydroperoxy endoperoxide (Prostaglandin G2; PGG2), and the peroxidase component reduces the endoperoxide to the corresponding alcohol (prostaglandin H2; PGH2), the precursor of prostaglandins, thromboxanes, and prostacyclins. It is now well established that there are two distinct isoforms of cyclooxygenase. COX-1 is constitutively expressed in a variety of cell types and is involved in normal cellular homeostasis. A variety of mitogenic stimuli such as phorbol esters, lipopolysaccharides, and cytokines lead to the induced expression of a second isoform of COX, COX-2. COX-2 is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions. This inducible COX-2 is believed to be the target enzyme for the anti-inflammatory activity of nonsteroidal anti-inflammatory drugs.|
|Storage :||Stability: 1 year;Storage: -80°C;This kit will perform as specified if stored as directed and used before the expiration date indicated on the outside of the box.|
◆ Assay kits
|Kit-0256||Cyclooxygenase 1 Inhibitor Screening Fluorometric Kit||+Inquiry|
|Kit-1941||COX Colorimetric Inhibitor Screening Assay Kit||+Inquiry|