"COX" Related Products

COX Activity Assay Kit

Cat.No.: Kit-0252
Product Overview: COX Activity Assay Kit measures the peroxidase activity of COX. The peroxidase activity is assayed colorimetrically by monitoring the appearance of oxidized N,N,N",N"-tetramethyl-p-phenylenediamine (TMPD) at 590 nm. It can be used with both crude (cell lysates/tissue homogenates) and purified enzyme preparations. The kit includes isozymespecific inhibitors for distinguishing COX-2 activity from COX-1 activity.
Description: Cyclooxygenase (COX, also called prostaglandin H Synthase or PGHS) is a bifunctional enzyme exhibiting both cyclooxygenase and peroxidase activities. The cyclooxygenase component converts arachidonic acid to a hydroperoxy endoperoxide (Prostaglandin G2; PGG2), and the peroxidase component reduces the endoperoxide to the corresponding alcohol (prostaglandin H2; PGH2), the precursor of prostaglandins, thromboxanes, and prostacyclins. It is now well established that there are two distinct isoforms of cyclooxygenase. COX-1 is constitutively expressed in a variety of cell types and is involved in normal cellular homeostasis. A variety of mitogenic stimuli such as phorbol esters, lipopolysaccharides, and cytokines lead to the induced expression of a second isoform of COX, COX-2. COX-2 is responsible for the biosynthesis of prostaglandins under acute inflammatory conditions. This inducible COX-2 is believed to be the target enzyme for the anti-inflammatory activity of nonsteroidal anti-inflammatory drugs.
Usage: Please read these instructions carefully before beginning this assay.For research use only. Not for human or diagnostic use.
Storage: Stability: 1 year;Storage: -80°C;This kit will perform as specified if stored as directed and used before the expiration date indicated on the outside of the box.
Kit Components: Kit components may be stored at -80°C prior to use. After opening kit, we recommend each kit component be stored according to the temperature listed below.Assay Buffer (10X): 1 vial, -20°C;Heme: 1 vial, -80°C;COX Standard: 1 vial, -80°C;Arachidonic Acid (substrate): 1 vial -20°C;Potassium Hydroxide: 1 vial, -20°C;Colorimetric Substrate: 1 vial, -20°C;DuP-697 Assay Reagent: 1 vial, -20°C;SC-560 Assay Reagent: 1 vial, -20°C;96-Well Plate: 1 plate, Room temperature;96-Well Cover Sheet: 1 cover, Room temperature

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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