Cytochrome P450 2C19 Inhibitor Screening Kit

PRODUCTS

Home / Products / Assay Kits / Activity Kits / Cytochrome P450 2C19 Inhibitor Screening Kit

Cytochrome P450 2C19 Inhibitor Screening Kit

CYP2C19 Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat.No. : Kit-2125
Product Overview : CYP2C19 Inhibitor Screening Kit enables rapid screening of drugs and other new chemical entities (NCEs) for compound-CYP2C19 interaction in a reliable, high-throughput fluorescence-based assay. The kit provides a yeast microsomal preparation of human CYP2C19 and cytochrome P450 reductase (CPR) enzymes. The assay utilizes a non-fluorescent CYP2C19 substrate that is converted into a highly fluorescent metabolite detected in the visible range (Ex/Em = 406/468 nm), ensuring a high signal-to-background ratio with little interference by autofluorescence. The kit contains a complete set of reagents sufficient for performing 100 reactions in a 96-well plate format.
Size : 100 assays
Applications : Rapid, high-throughput screening of drugs and novel ligands. Development of structure-activity relationship (SAR) models to predict CYP2C19 inhibition liability of novel compounds.Prediction of adverse drug-drug interaction potential and bioavailability for compounds metabolized by CYP2C19.
Storage : Store kit at -20°C and protected from light. Briefly centrifuge all small vials prior to opening. Allow the CYP2C19 Assay Buffer to warm to room temperature (RT) prior to use. Read entire protocol before performing the assay procedure.3-CHC Standard: Reconstitute in 110 µl of DMSO and vortex until fully dissolved to yield a 5 mM stock solution. The 3-CHC stock solution should be stored at -20°C and is stable for at least 3 freeze/thaw cycles.CYP2C19 Inhibitor (Ticlopidine): Reconstitute in 220 µl of deionized H2O and vortex until fully dissolved to yield a 10 mM stock solution. The stock solution is stable for 2 months at -20°C. To obtain a 150 µM working solution of ticlopidine (5X final concentration), add 15 µl of the 10 mM stock to 985 µl of CYP2C19 Assay Buffer. Store resulting 150 µM solution at -20°C and use within one week.NADPH Generating System (100X): Reconstitute with 110 µl CYP2C19 Assay Buffer, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. Keep on ice while in use.β-NADP+ Stock (100X): Dissolve in 110 µl CYP2C19 Assay Buffer and vortex thoroughly (100X stock). Store at -20°C, stable for at least 3 freeze/thaw cycles.CYP2C19 Substrate: Reconstitute with 110 µl anhydrous HPLC-grade acetonitrile and vortex until fully dissolved to obtain a 5 mM stock solution. Store at -20°C. When using the CYP2C19 Substrate stock solution, allow the vial to warm to RT before opening and promptly retighten cap after use to avoid absorption of airborne moisture.Recombinant Human CYP2C19: The Recombinant Human CYP2C19 should be reconstituted immediately before use as directed in Protocol.2 below. Each vial is sufficient for preparation of 50 reactions in a 96-well plate format.

Products Types

◆ Recombinant Protein

CYP2C19-126CRecombinant Cynomolgus CYP2C19 Protein+Inquiry
CYP2C19-75HRecombinant Human CYP2C19 Protein+Inquiry
CYP2C19-33HRecombinant Human CYP2C19 Protein+Inquiry
CYP2C19-541HRecombinant Human CYP2C19+Inquiry
CYP2C19-540HRecombinant Human CYP2C19 (I331V)+Inquiry

See All CYP2C19 Recombinant Protein

◆ Assay kits

Kit-2124Cytochrome P450 2C19 Fluorometric Activity Assay Kit+Inquiry

See All CYP2C19 Assay kits

Related Gene

CYP2C19CYP2C9CYP2D6CYP2E1CYP3A4CYP1B1CYP24A1CYP2J2CYP46A1CYP7B1CYP1A2CYP19A1CYP26A1CYP26B1CYP2A6CYP3A5CYP3A7CYP4F11CYP4F3CYP7A1CYP2C6CYP3A23/3A1CYP1A1CYP2B6CYP4A11Cyp11a1CYP2C8CYP2D4CYP3A2CYP2C18CYP3A43CYP4B1CYP2U1CYP2W1CYP39A1CYP2D1CYP2R1CYP26C1CYP27A1CYP2S1CYP2C11CYP4V2CYP4X1CYP4Z1CYP11B1CYP19A1BCYP4F12CYP4F2CYP4F22CYP38CYP27B1CYP27C1CYP2A13CYP2A7CYP20A1CYP21A2CYP4F8CYP17A1CYP2C23CYP4A22CYP4F13Cyp2a1Cyp2a12Cyp2b3Cyp2b9Cyp2c12Cyp2c13Cyp2c22Cyp2c29Cyp2c38Cyp2c39Cyp2c40Cyp2c44Cyp2c50Cyp2c54Cyp2d11Cyp2d12Cyp2d2Cyp2d22Cyp2d26Cyp2d3Cyp2d34Cyp2d40Cyp2d5Cyp2d9Cyp2f2Cyp2f4Cyp2g1Cyp2c37Cyp21a1Cyp3a11Cyp3a13Cyp3a16Cyp3a18Cyp3a25Cyp3a41aCyp3a41bCyp3a57Cyp4a31Cyp4a32Cyp4a8Cyp4f1Cyp4f14Cyp4f15Cyp4f17Cyp4f18Cyp4f6Cyp4v3Cyp51CYP8B1Cyp11b2Cyp11b3Cyp2a2Cyp2a22Cyp2a3Cyp2a4Cyp2a5Cyp2ab1Cyp2b1Cyp2b10Cyp2b12Cyp2b13Cyp2b15Cyp2b19Cyp2b21Cyp2b23Cyp2c6v1Cyp2c65Cyp2c66Cyp2c67Cyp2c68Cyp2c69Cyp2c7CYP2F1Cyp2j10Cyp2j12Cyp2j13Cyp2j4Cyp2j8Cyp2j9Cyp2t1Cyp2t4Cyp3a23Cyp3a44Cyp3a62Cyp3a9Cyp4a1Cyp4a12aCyp4a12bCyp4a14Cyp4a2Cyp4a29Cyp4a3Cyp4a30bCyp4f39Cyp4f4Cyp4f40Cyp4f5CYP51A1Cyp3a59CYP1B1-AS1CYP2B7P1

Online Inquiry


  • Note: There will be extra charge for optional service!
  • Verification Code Please review Creative BioMart's privacy policy for more information

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2007 – 2021 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy