Recombinant Human Polo-like Kinase 3 (Drosophila), His-tagged PLK3-566H

Cat. No.:	PLK3-566H
Product Overview:	Recombinant Human Plk3 (a.a. 58-339) with N-terminal His tag expressed in aBaculovirus infected Sf9 cellexpression system. MW=36.7 kDa.
Description:	Polo-like kinase 3 (Drosophila), also known as PLK3, is an enzyme which in humans is encoded by the PLK3 gene. Cytokine-inducible kinase is a putative serine/threonine kinase. CNK contains both a catalytic domain and a putative regulatory domain. It may play a role in regulation of cell cycle progression and tumorigenesis. PLK3 has been shown to interact with CDC25C, CHEK2 and P53.
Source:	Baculovirus infected Sf9 cells.
Application:	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Specific Activity:	500 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of casein substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP.
Purity:	>80% by SDS-PAGE.
Formulated In:	25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Stability:	>6 months at -80°C.

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Gene Name:	PLK3 polo-like kinase 3 (Drosophila) [ Homo sapiens ]
Synonyms:	polo-like kinase 3 (Drosophila); CNK; FNK; PRK; PLK3; serine/threonine-protein kinase PLK3; PLK-3; FGF-inducible kinase; cytokine-inducible kinase; proliferation-related kinase; cytokine-inducible serine/threonine-protein kinase; Polo-like kinase 3; Proliferation-related kinase; Cytokine-inducible serine/threonine-protein kinase; EC 2.7.11.21
Gene ID:	1263
mRNA Refseq:	NM_004073.2
Protein Refseq:	NP_004064.2
MIM:	602913
UniProt ID:	Q9H4B4
Chromosome Location:	1p34.1
Function:	ATP binding; nucleotide binding; polo kinase kinase activity; protein binding; protein serine/threonine kinase activity; transferase activity