||Recombinant Human Plk3 (a.a. 58-339) with N-terminal His tag expressed in aBaculovirus infected Sf9 cellexpression system. MW=36.7 kDa.
||Polo-like kinase 3 (Drosophila), also known as PLK3, is an enzyme which in humans is encoded by the PLK3 gene. Cytokine-inducible kinase is a putative serine/threonine kinase. CNK contains both a catalytic domain and a putative regulatory domain. It may play a role in regulation of cell cycle progression and tumorigenesis. PLK3 has been shown to interact with CDC25C, CHEK2 and P53.
||Baculovirus infected Sf9 cells.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
||500 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of casein substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP.
||>80% by SDS-PAGE.
||25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
||>6 months at -80°C.